Eppendorf pcr consumables compatibility guide for pcr and qpcr cyclers rafal grzeskowiak1, sandrine hamels2, beate riekens1 1 eppendorf ag, hamburg, germany. Realtime pcr detection systems life science research. Hence, the time it takes to complete an assay is decreased. Being a newcomer to realtime pcr, i trialled a few different instruments. Mastercycler ep realplex realtime pcr system eppendorf. Allelic discrimination introduction getting started guide. Heatsealer pcr accessories, pcr eppendorf middle east. Mastercycler realplex2 from eppendorf biocompare product. Because of its importance and widespread use, several companies offer real time pcr platforms that meet the latest quantitative pcr applications. A combination of special manufacturing technologies and selected polypropylene batches ensures nearly 100 % recovery of dnarna moleculeswithout surface coating to eliminate the risk of sample contamination. The limiting factor in low volume real time pcr is. Make offer eppendorf mastercycler 5333 pcr thermal cycler. Other features include the ability to send programs to all or only a few mastercycler pro at a time, glpconformity documentation of all pcr experiments, and real time acquisition of temperature data.
Copy and save results to special software tools for relative quantification. Films and foils provide your pcr plate with a tight seal, particularlty when heatsealing material is used. Eppendorf limited product warranty mastercycler ep. Eppendorf pcrcooler for safe cooling of reagents at the bench. Eppendorf pcr consumables compatibility guide for pcr and.
Pcr films and foils pcr consumables, pcr eppendorf. Its flexible enough for any real time assay, and can deal with multiplexing and snps easily. Cfx maestro software is built to work with the cfx series of real time pcr systems and is an easytolearn and easytouse comprehensive tool for plate setup, data collection, data analysis, and data visualization of real time pcr. Eppendorf realplex4 96 well 4 color realtime pcr systemyear.
Eppendorf mastercycler ep realplex instrument setup instructions for rt2 profiler pcr arrays. The tubestrips are suitable for fast endpoint pcr and real time pcr applications. Roche cobas taqman 96 automated real time pcr amplification detector analyzer. Eppendorf has developed a realtime pcr system that meets the requirements. Eppendorf mastercycler ep realplex realtime pcr system. However, it can be difficult when you want to do something that you. Eppendorf mastercycler ep realplex instrument setup instructions for. Adsorption of dna to tube surfaces effects accuracy of real time pcr results. Satellite system use allows users to control up to five units with one control panel or to expand to thirty units with a computer network using cyclemanager pro software. Eppendorf pcr consumables compatibility guide for pcr. Eppendorf microloader eppendorf pcr tubes reagent reservoirs tiptub transfertip r f rp es twin. Founded and staffed by people with a passion for technology, for over 10 years itis has been delivering high performance technology for real time pcr.
This graph indicates there is up to a 10fold higher fluorescence measurement when using twin. Available realtime rtpcr cycler and kinetic pcr machines. You can choose from a more compact s100 and a more flexible s200 instrument. The eppendorf pcr tubes are easy to open, but provide tight sealing to prevent evaporation in pcr. Eppendorf enewsletter subscribe to our email newsletter and you wont miss.
The machine features, like some other mastercyler devices from eppendorf, a special 96well heating block, which enables rapid thermal cycling. Instrument is also an licensed realtime thermal cycler for use with. Easy installation and deinstallation and compatible with windows. The fast pcr tube strip allows scientists using fast cyclers such as the mastercycler x50 and fast enzymes to obtain higher pcr yield. Product registration register your product and receive up to 100 bonus eppoints.
It illustrates the usefulness of absolute and relative quantification assays in real time pcr and real time rt pcr. Heatsealer pcr accessories, pcr eppendorf hong kong. The eppendorf heatsealer family offers you reliable and effective sealing of a variety of plates. I also think it represents good value for money in terms of its features, and quick cycling time. Main focus of gene quantification web page is to describe and summarize all technical aspects involved in quantitative gene expression analysis using real time rt pcr and competitive rt pcr. This is achieved by the faster heat transfer from block to reaction, allowing for better control over the reaction. Thats when you need high quality pcr cyclers and consumables to eliminate unwanted variables from your assays. Find and purchase eppendorf mastercycler realplex products at invitrogen life science technologies. Eppendorf pcr tubes tubes, laboratory consumables pcr. With the eppendorf software, it is very easy to program in an assay. On this page the most prominent realtime pcr cycler are described.
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